Research (Scientific and Technical -The post holder will be central to the collection and processing of biological samples collec.....
Research (Scientific and Technical -The post holder will be central to the collection and processing of biological samples collected as part of this prospective study. They will work alongside a team of clinical research nurses and study coordinators at Kings College Hospital. In addition, they will be responsible for the transfer of samples and data to AstraZeneca laboratories. Responsible for designing, planning, and executing experiments as part of the translational research study focusing on SLE. Conduct a number of experimental assays, including developing and running cell phenotype experiments (namely with CyTOF, flow cytometry, RNAseq and spatial transcriptomics). In addition to conducting real-time PCR, microarrays, ELISA and cell culture experiments. Previous experience of these techniques is desirable. Utilize multiomics approaches, including genomics, transcriptomics, proteomics, and metabolomics, to comprehensively analyze molecular signatures associated with SLE. Analyze experimental data using bioinformatics tools and statistical methods to derive meaningful insights into the pathogenesis of SLE. Process research samples from whole blood and tissue. Participate in all manual, semi-automated and fully automated laboratory investigations. Maintain and operate equipment and instruments used by the research group, including management of stock (such as laboratory reagents). Organise shipment of samples to collaborators. Maintain accurate records of experimental procedures, results, and findings. Prepare research manuscripts for publication in peer reviewed journals and abstracts for presentation at national and international conferences. Clinical Ensure adequate and accurate recording of all clinical data on the secured study database. Assist in participant recruitment to translational studies. Tasks such as phlebotomy may be required (training can be provided if not currently able to perform task). Pursue Good Clinical Practice, Human Tissue Act and consent training. Assist in quality control audits relating to the research study. Administrative Plan timing of biological sample collection and facilitate study visits by liaising with relevant members of clinical and biobanking teams. Ensuring adherence to standard operating procedures (SOPs) and ethical guidelines are maintained across research activities relating to this study. Assist in the generation of study relevant paperwork, including ethical applications and grant writing. Communicate effectively at all times with all members of the study team and participants. Work alongside the study team to identify potential participants for the study. Maintain a safe working environment in accordance with the Health and Safety at Work Act. Ensure that staff have completed relevant training within the laboratory research team. Provide assistance in ordering relevant consumables required to run the study and that supplies are adequately stocked. Ensure laboratory equipment is in good working order and report any defects appropriately. Maintain accurate documentation in accordance with the Trust policies, particularly in relation to expected / stored samples. Assisting with the secure sharing of data with other parties involved in the research study. Ensure the study is conducted in compliance with Good Clinical Practice and the Human Tissue Authority guidelines. Education and Training Provide training to junior members of the laboratory team (including PhD students, clinical research fellows and MSc students). If interested, supervisor roles of MSc students within KCL is possible. Maintaining an up-to-date knowledge of the pathogenesis of systemic lupus erythematosus and relevant immunological techniques. Attend local, national and international meetings to present findings of this research. Opportunities for teaching clinician on immunology will be available, if of interest. Collaborate effectively with multidisciplinary team members, including clinicians, bioinformaticians, and other researchers, to achieve project goals. Other Understand and adhere to Trust policies and procedures. Maintain patient confidentiality and standards of Good Clinical Practice (GCP) and ensure compliance with regulatory standards, Good Laboratory Practice (GLP), and contribute to quality assurance activities. Comply with both local and national standards for safe, secure and confidential processing and storage of patient and laboratory information. Comply with departmental procedural policies on accessing patient related information on the study database. Develop and maintain research results database. Autonomously work within the department operating procedures and protocols. Maintain good working relationships between keen stakeholders in this research study including the Sponsor (Kings College Hospital), the Funder (AstraZeneca), the laboratory team (based at the James Black Centre), and rheumatology study teams based at Kings College Hospital and Guys and St. Thomas Hospitals.
